Input Sequences: Enter the DNA sequence you want to edit in the "Target Sequence" field. Enter the guide RNA (gRNA) sequence in the "gRNA Sequence" field. This sequence helps the CRISPR system find the target DNA. Enter the "Edit Sequence" which is the sequence that will replace the target sequence.
Simulate Edit: As you type in the sequences, the tool will automatically update and simulate the CRISPR edit. The tool will use the gRNA to locate the target sequence and make a cut, simulating an edit.
View Results: The original and edited sequences are displayed side by side. The impact of the edit on specific gene regions is also visualized.
The default example values are pre-populated to help you understand how the tool works:
In this example, both TAGG gRNA are located and replaced with Edit Sequence: TCGG, resulting in the edited sequence "AGCTTCGGCTA-GATCGGCTATC".
Currently, the application is basic and serves as an educational tool for understanding CRISPR mechanics. Here are some known issues and areas for improvement:
For a more comprehensive CRISPR simulation experience, consider exploring dedicated bioinformatics tools and software platforms.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology allows scientists to make precise changes to the DNA of living organisms. It uses a guide RNA (gRNA) to find a specific sequence in the DNA. The Cas9 enzyme, guided by the gRNA, makes a cut at this location, enabling the introduction, removal, or alteration of DNA segments. This tool simulates this editing process, providing a visual representation of how specific DNA sequences can be altered using CRISPR.
This powerful technology has applications in research, medicine, and biotechnology, including gene therapy, agriculture, and disease study. For more information, you can refer to the following resources: